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上海miRNA测序实验

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上海miRNA测序实验

miRNA CEXUSHIYANJIUTEDINGZUZHIZAITEDINGZHUANGTAIXIADEYIZHImiRNA,FENXIYUCEXINDEmiRNA,BINGDUIYIZHI miRNA HEXINmiRNAJINXINGBAJIYINYUCE。TONGGUODUImiRNABAJIYINZHUSHIJIDAIXIETONGLUFENXI,JIANGJIANDINGCHUDE miRNAYUXIANGYINGBIAOXINGCHAYIGUANLIANQILAI,WEIYANJIUmiRNADUIXIBAOJINCHENGDEZUOYONGJIQISHENGWUXUEYINGXIANGTIGONGLEYOULIDEGONGJU。

上海miRNA测序实验生物信息分析内容

1、SHUJUCHANCHUTONGJIHEZHILIANGPINGGU

2、sRNAZAICANKAOJIYINZUSHANGDEFENBU

3、sRNAFENLEIZHUSHITONGJI

4、JIANJIPIANHAOXINGFENXI

5、JIYINBIAODASHUIPINGFENXI

6、XINZHUANLUBENYUCE

7、CHAYImiRNABAJIYINYUCE

8、CHAYIJIYINSHAIXUANJIJULEIFENXI

9、CHAYIBIAODAmiRNABAJIYINGOFUJIFENXI

10、CHAYIBIAODAmiRNABAJIYINPathwayFUJIFENXI

11、CHAYIBIAODAmiRNABAJIYINDANBAIHUZUOWANGLUOFENXI

 

技术参数

YANGBENLEIXING:total RNA

YANGBENYAOQIU:ZONGLIANG≥3 μg,NONGDU≥200 ng/μL

WENKULEIXING:Small RNAWENKU

CEXUCELVE:HiSeq SE50,TUIJIANSHUJULIANG:≥10M reads

XIANGMUZHOUQI:35GEGONGZUORI

 

结果示例

YANGBENYIZHImiRNAGEWEIZHIJIANJIPIANHAOXING

 

XINmiRNAERJIJIEGOUJIreads mappingSHIYITU

 

GEGEYANGBENZHIJIANGONGYOUnovel miRNAFENBU

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